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      Power-Bind 链霉亲和素包被的聚苯乙烯微粒

      Power-Bind非磁性链酶亲和素包被微粒可改进并简化微粒与基团的结合。它们结合了表面积大以及简单、亲和力高与特异性高的优势。它们也可应用于一系列诊断与分子生物学领域。这些单一分散的微粒悬浮液,以高活性的链霉亲和素共价结合于Opti-Link羧基修饰微粒表面为特征,具有很高的生物素结合能力与很长的效期。众所周知,这些微粒可用作各种免疫分析与亲和纯化的固相支持物。但是,要取得固相基团的高活性与稳定

      1. 详细信息

      Power-Bind 非磁性链酶亲和素包被微粒可改进并简化微粒与基团的结合。它们结合了表面积大以及简单、亲和力高与特异性高的优势。它们也可应用于一系列诊断与分子生物学领域。
      这些单一分散的微粒悬浮液,以高活性的链霉亲和素共价结合于Opti-Link 羧基修饰微粒表面为特征,具有很高的生物素结合能力与很长的效期。

      众所周知,这些微粒可用作各种免疫分析与亲和纯化的固相支持物。

      但是,要取得固相基团的高活性与稳定的结合力是一个很大的挑战。在缓冲液中经过简单孵育后,生物素化的复合物很容易稳定结合于Power-Bind链酶亲和素微粒。这主要是由于生物素与链霉亲和素间相互作用的高亲和性。
      链酶亲和素可起到空间隔离的作用,这可以提高结合基团的特异反应性。
      Power-Bind 链酶亲和素微粒符合诊断试剂生产商与核酸研究工作者的需求。
      因为众所周知的生物素-链霉亲和素反应,科学工作者们可容易地将基团结合于微粒表面。
      用传统的方法很难与微粒表面结合的复合物,却可被生物素化。其中包括活化反应必须在有机溶剂中进行的复合物。对于此种复合物,生物素化可在有机溶剂中进行。随后, 生物素衍生物即可与 Power-Bind 链霉亲和素微粒简单地混匀。
      很难吸附于微粒表面的核酸,经过生物素化后,即可很容易地结合于Power-Bind 链酶亲和素微粒。

       

      优势
      • 解离常数(Kd 10-15 摩尔)
      • 配基结合稳定
      • 生物素化配基的简单的一步法结合方案
      • 表面结合基团的高活性
      • 耦合难题的轻松解决
      • 简单的溶液中生物素化反应
      • 非特异干扰反应低
      • 微粒活动性高,适用于基于膜的应用领域
      • 采用生物素/酶检测系统可应用于 EIA 设计中
      • SA 分子有利的空间效应
      • 根据不同的应用需求,可选择0.3 μm 或 0.8 μm直径的微粒
      • 适用于生物学应用领域的高生物素结合能力

       

      Bind a variety of biotinylated ligands, including DNA/RNA, oligos, antibodies and proteins with Thermo Scientific™ Power-Bind Streptavidin Coated Particles. Particles have very high and stable biotin binding capacities and address the concerns of diagnostic test kit manufacturers and nucleic acid researchers. The microparticles reduce serum matrix effects and improve assay accuracy, resulting in low, non-specific binding of serum proteins and other interfering substances.

      The use of microparticles as a solid phase support in various immunoassays and affinity purifications has been known for some time; however, achieving high activity and stable binding of solid phase ligands has been a major difficulty. Biotinylated compounds become stably bound to Power-Bind Streptavidin Coated particles after simple incubation in buffer due to the high affinity of the biotin-streptavidin interaction. The particles also function as a spacer, which improves the specific activity of the bound ligand. Monodisperse nonmagnetic particle suspensions with streptavidin covalently bound to the surface in a highly active form

      Features:

      • Made from 0.3μm or 0.8μm Thermo Scientific™ OptiLink™ Carboxylate-Modified particles
      • High biotin-binding capacity
      • Extremely stable, long shelf life
      • Simplifies the process of binding ligands to particles
      • Combines the advantages of high surface area and easy, high affinity, high specific activity binding
      • Excellent for chemiluminescent assays, biotinylated ligand capture, and for molecular biology, immunoassay, or sample prep applications

      Benefits:

      • Dissociation constant (Kd 10-15 molar), easy one-step binding protocols for biotinylated ligands
      • High activity of surface bound ligands to easily solve difficult coupling problems
      • Simple aqueous biotinylation reactions
      • Low nonspecific interactions (blocked microparticle surface)
      • Highly mobile particles for membrane-based applications
      • Can be used in EIA formats with biotin/enzyme detection systems
      • Beneficial spacer effect of SA molecule
      • A choice of 0.3μm or 0.8μm diameters for different application requirements

      Recommended for:

      • Universal nonmagnetic bead for coupling unique ligands.
      • Clinical diagnostics and molecular biology applications.

       

      目录号
      数量
      直径(公制) 颗粒
      29000701010150
      5mL
      0.3µm
      描述 Power-Bind Streptavidin Coated Particles
      直径(公制) 颗粒 0.3µm
      包括 Package Insert and Certificate of Analysis
      数量 5mL
      样品评估包 Power-Bind evaluation samples are packaged in 1mL bottles at 1% solids (10 mg/mL). Select individual lots to evaluate.
      有效期 24 Months
      专用包装 Power-Bind Streptavidin Coated particles are available in different concentrations and package sizes upon request.
      稳定性 Guanidinium thiocyanate; DMF and DMSO to 100% Sonication; environments PCR temperature cycling
      储存要求 2° to 8°C
      防腐剂 0.05% Na Az
      认证/合规 Includes Certificate of Analysis
      29000701010350
      100mL
      0.3µm
      描述 Power-Bind Streptavidin Coated Particles
      直径(公制) 颗粒 0.3µm
      包括 Package Insert and Certificate of Analysis
      数量 100mL
      样品评估包 Power-Bind evaluation samples are packaged in 1mL bottles at 1% solids (10 mg/mL). Select individual lots to evaluate.
      有效期 24 Months
      专用包装 Power-Bind Streptavidin Coated particles are available in different concentrations and package sizes upon request.
      稳定性 Guanidinium thiocyanate; DMF and DMSO to 100% Sonication; environments PCR temperature cycling
      储存要求 2° to 8°C
      防腐剂 0.05% Na Az
      认证/合规 Includes Certificate of Analysis
      29000701011150
      1mL
      0.3µm
      描述 Power-Bind Streptavidin Coated Particles
      直径(公制) 颗粒 0.3µm
      包括 Package Insert and Certificate of Analysis
      数量 1mL
      样品评估包 Power-Bind evaluation samples are packaged in 1mL bottles at 1% solids (10 mg/mL). Select individual lots to evaluate.
      有效期 24 Months
      专用包装 Power-Bind Streptavidin Coated particles are available in different concentrations and package sizes upon request.
      稳定性 Guanidinium thiocyanate; DMF and DMSO to 100% Sonication; environments PCR temperature cycling
      储存要求 2° to 8°C
      防腐剂 0.05% Na Az
      认证/合规 Includes Certificate of Analysis
      29001801010150
      5mL
      0.85µm
      描述 Power-Bind Streptavidin Coated Particles
      直径(公制) 颗粒 0.85µm
      包括 Package Insert and Certificate of Analysis
      数量 5mL
      样品评估包 Power-Bind evaluation samples are packaged in 1mL bottles at 1% solids (10 mg/mL). Select individual lots to evaluate.
      有效期 24 Months
      专用包装 Power-Bind Streptavidin Coated particles are available in different concentrations and package sizes upon request.
      稳定性 Guanidinium thiocyanate; DMF and DMSO to 100% Sonication; environments PCR temperature cycling
      储存要求 2° to 8°C
      防腐剂 0.05% Na Az
      认证/合规 Includes Certificate of Analysis
      29001801010350
      100mL
      0.85µm
      描述 Power-Bind Streptavidin Coated Particles
      直径(公制) 颗粒 0.85µm
      包括 Package Insert and Certificate of Analysis
      数量 100mL
      样品评估包 Power-Bind evaluation samples are packaged in 1mL bottles at 1% solids (10 mg/mL). Select individual lots to evaluate.
      有效期 24 Months
      专用包装 Power-Bind Streptavidin Coated particles are available in different concentrations and package sizes upon request.
      稳定性 Guanidinium thiocyanate; DMF and DMSO to 100% Sonication; environments PCR temperature cycling
      储存要求 2° to 8°C
      防腐剂 0.05% Na Az
      认证/合规 Includes Certificate of Analysis
      29001801011150
      1mL
      0.85µm
      描述 Power-Bind Streptavidin Coated Particles
      直径(公制) 颗粒 0.85µm
      包括 Package Insert and Certificate of Analysis
      数量 1mL
      样品评估包 Power-Bind evaluation samples are packaged in 1mL bottles at 1% solids (10 mg/mL). Select individual lots to evaluate.
      有效期 24 Months
      专用包装 Power-Bind Streptavidin Coated particles are available in different concentrations and package sizes upon request.
      稳定性 Guanidinium thiocyanate; DMF and DMSO to 100% Sonication; environments PCR temperature cycling
      储存要求 2° to 8°C
      防腐剂 0.05% Na Az
      认证/合规 Includes Certificate of Analysis