Improved Array Method for Size Calibration of Monodisperse Spherical Particles by Optical Microscope
- 更新时间2015-02-09 13:37:43
Monodisperse or highly uniform spheres, when placed on a fl at surface in a liquid medium, align themselves into systematic hexagonal arrays characterized by straight rows of particles. Using an optical microscope, the length of a row can be measured and divided by the
number of spheres in the row to calculate the average diameter of the spheres. Limitations of the traditional array methods have been avoided by improved sample preparation methods and careful selection of measurement rows. Using the improved method, a series of monodisperse spherical particles from 0.5 to 40 micrometers (μm) was calibrated and certifi ed with a stage micrometer calibrated by the National Institute of Standards and Technology (NIST).