Thermo Particle Technology

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    Internal Standard Method for Size Calibration of Sub-Micrometer Spherical Particles

    • 更新时间2015-02-09 13:23:31

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    Transmission Electron Microscopy (TEM) is an accepted method for measuring the mean diameter and size distribution of polymer latex microspheres1,2. However, errors in the size data of uniform latex particles from commercial suppliers3, and the need for traceability of size data to the National Bureau of Standards (NBS)4, raise doubts about the suitability of TEM size data for calibrating spherical size standards.
    In addition to needing accurate data, the authors’laboratory needed to calibrate smaller than usual latex microspheres. By omitting several sources of error in typical TEM calibration procedures5,6 and by modifying the sample preparation procedure, we were able to make improvements in the method. This enabled us to calibrate a new series of polymer microspheres for use as certifi ed particle size standards
    from 50 nanometers (nm) to 1 micrometer (μm).